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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was identified to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant purpose in promoting cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up noted but in-depth nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged with the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is particular.
Concentrate on Facts Cyclin E belongs towards the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress as a result of thr77 login S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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